However, fortunately, the preamp tube closest to the input is almost always the V1, as this also means the shortest signal path.
The V1 are the first gain stages (each preamp tube can have two gain stages); because it is the first it has the biggest impact on tone and noise and should be of good quality and low noise. The other gain stages (V2 and V3) usually are not that important anymore, at least to my mediocre ears.
The PI should be a balanced preamp tube. Depending on your amp you might also have a tube buffered effects loop.
For example, if I remember correctly, a Peavey 5150 has 5 preamp tubes - 3 are gain stages (I think 5 or 6 in total), one is for the effects loop and one is the PI.