It's very likely to be a tube. You'll need a known good 12ax7 to swap in for each of the pre-amp tubes in term, to find out if it's one of them that's faulty. Otherwise, you'll need to try another set of EL34's - if it's just for a minute or so, you won't need to bias them, so you can just borrow a "mate's" set if you want to try them out - just keep an eye out for any red-plating if you're quickly trying any out without re-biasing.