I personally like to have the HT fuse after the first filter cap from the rectifer, and use a fast blow fuse. 1A would be a HUGE fuse to use here in a 100W amp, as the HT is probably at least 450 VDC, so you would need be pulling at least 450W through the power valves for the fuse to blow!
I like to have the HT fuse after the first filter cap (and before the OT centre tap obviously) because, in my experience, bias failure and valves shorting out (especially) are relatively common occurances, and will cause a fuse located here to blow, ie do its job and prevent any collateral damage to either of the transformers.
Some amps have their HT fuse before the first filter cap, or before the rectifier, in which case you will need a much larger and slow blow fuse to accomadate the fact that the first filter cap is charged in pulses from the rectifier. These pulses are usually about 5-10 times the static current draw of the amp.
The arguement for having the fuse before the first filter cap and/ or rectifier is that if the cap or rectifer shorts out then the fuse will blow and save the mains transformer. Again from my experience, if the rectifier or main filter caps go short circuit, then the amp's mains fuse will blow and save the mains transformer, however bias failure (usually) won't blow an adequately rated fuse in this position.
I have to admit that this is one issue where I would disagree with Merlin Blencowe (
http://www.freewebs.com/valvewizard/fuses.html). I recommend you read his views on this and come to your own conclusions.
For the ultimate protection you probably would want a fast blow HT fuse after the first filter cap and a larger slow blow fuse/s before the rectifier.